Phytochemical screening of antibacterial and antioxidant activities of Lavandula multifida L

Lavandula multifida a medicinal plant from the Lamiaceae family is commonly used in

traditional medicine to address various ailments. The sustainable use and valorization of natural

resources are becoming increasingly significant concerns in our country. This study aims to perform

phytochemical screening and evaluate the antibacterial and antioxidant properties of Lavandula

multifida. Hydro-ethanolic extract sourced from Boulhaf Eddir in the Tebessa region (southeastern

Algeria). The extract was prepared through maceration using water and ethanol, yielding 38.57 %.

Phytochemical analysis revealed the presence of flavonoids, catechic tannins, gallic tannins,

saponins and steroids, while alkaloids, terpenes and quinones were absent. The quantitative

antibacterial assessment showed variability in inhibition zone diameters against the tested bacterial

strains, with the minimum inhibitory concentration (MIC) recorded at 12.25 mg/mL for S. aureus

and B. subtilis and 25 mg/mL for K. pneumoniae and E. coli. The CMB/MIC ratios indicated a

bactericidal effect of the extract on all tested strains. Additionally, the antioxidant activity assay

demonstrated significant DPPH radical scavenging capability, with IC50 values of 117.0 ±

3.46µg/mL for leaf extract and 100.2 ± 7.56µg/mL for flower extract. These promising results

suggest the potential application of Lavandula multifida extract in the agro-food and pharmaceutical

industries for its antibacterial and antioxidant properties.