Phytochemical screening and In-vivo/ In-vitro bioactivity of Rhamnus alaternus vs Tuta absoluta

Author(s) Chelef Mokhtaria, Mohdeb Samra, Bentoumi Abderrahmane and Mohammed Ait Hammou
Year: 2026
Month: May
Volume: 68
Pages: 75-94

In in-vitro and in-vivo bioassays, we assessed the insecticidal and antifeedant activity against of

aqueous extract of Rhamnus alaternus L. (Rhamnaceae) leaves against Tuta absoluta Meyrick

(Lepidoptera: Gelechiidae). We studie the insecticidal and antifeedant activity of aqueous (10.44

%) and ethanolic (12.9 7%) maceration extracts against Tuta absoluta and identified their

phytochemicals (Flavonoids, tannins, saponins, alkaloids, sterols and terpenoids). The in- vitro 20

% aqueous extract proved significantly lethal (100 %) and antifeedant (53.08  1.70 %)

bioinsecticide potential. 20 days after treatment, in in-vivo trials of curative, preventative and

reference treatments based on Chlorantraniliprole/ Abamectin showed mortality of 70 %, 54.8 %

and 96.4 %, respectively, with reduction in the damage index, showed quicker effects. The aqueous

extract demonstrated their lethal and anti-feedent bio-insecticide potential, ideal for integrated pest

management against T. absoluta, with fast action and an environmentally friendly profile.