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Quantitative analysis and evaluation of antioxidant activity of medicinal plant Moringa Oleifera L.

Quantitative analysis and evaluation of antioxidant activity of medicinal plant Moringa Oleifera L.

  • Author(s) Ruchi Agrawal, Yagyavalkya Sharma, Hemlata Bhatt, Vishal Khandelwal and Harsh Vardhan
  • Year: 2025
  • Month: January
  • Volume: 64
  • Pages: 25-36


The medicinal plant Moringa Oleifera L. leaf methanol extract was analyzed for its total polyphenol content, total saponin content, total phlobatannin content and total flavonoid content. Four in-vitro antioxidant assays [2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), metal chelating activity, and total antioxidant capacity (TAC). were done to measure the reducing power and the scavenging ability of the extract. The antioxidant analysis indicated that IC50 and percentage (%) inhibition were dose-dependent and showed the highest antioxidant activity (7.05 ± 0.2 µg/mL) for metal chelating ferrous ion activity followed by 2.54 ± 0.01 µg/mL for FRAP, 3.90 ± 0.02 µg/mL for DPPH, and 1.50 ± 0.02 µg/mL for TAC. Thus the methanol extract of Moringa oleifera leaf has great potential as natural natural therapy, anti-oxidant source, and for the conservation of raw and processed foods.



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