The study aimed to test the application of some endemic PGPR isolates on the cultivation of tomato hybrid variety "Tsarine" to improve its growth, production and fruit quality. We used 12 endemic bacterial PGPR isolates of Pseudomonas genus, from these bacterial suspensions were prepared and sprayed on seeds, then irrigated the young seedlings separately with each prepared bacterial suspension until the flowering. The isolate AC6 stimulated plant height and leaf biomass.