Herbicidal activity of Ageratum conyzoides L. on Amaranthus spinosus L. and GC-MS profile of n-hexane extract
Herbicidal activity of Ageratum conyzoides L. on Amaranthus spinosus L. and GC-MS profile of n-hexane extract
- Author(s) G. Erida, M. Hayati, T. Kurniawan and T.M. Rizki
- Year: 2025
- Month: July
- Volume: 65
- Pages: 159-174
We evaluated the herbicidal activity of sub-subfractions recovered from n-hexane leaf extract of Ageratum conyzoides L. on Amaranthus spinosus L. growth. The column chromatography was used to separate the n-hexane extract into its subfractions (A) and rechromatograph into its two sub-fractions, sub-subfractions A1 and sub-subfractions A2. In pot culture, these sub-subfractions were applied to A. spinosus at 2 %, 4 %, 6 % and 8 % concentrations and distilled water as control. The sub-subfractions showed phytotoxic activity one day after application. All sub-subfractions had promising herbicidal effects on A. spinosus as they suppressed A. spinosus growth by 100 % at 2 % concentration or higher in 1 day after application (DAA). The compounds detected by GC-MS in sub-subfraction A1 were 2H-1-benzopyran, 6,7-dimethoxy-2,2-dimethyl- (11.01 %), caryophyllene oxide (10.27 %), phytol (8.79 %), cyclohexene, 3-(1,5-dimethyl-4-hexenyl)-6-methylene-, [S-(R*,S*)]-(7.32 %), caryophyllene (7.23 %), phenol, 2,6-bis(1,1-dimethylethyl)-4-ethyl- (5.56 %), 1-octadecanethiol (CAS) octadecanethiol (4.71 %), squalene (4.5 %), phytol, acetate (4.17 %), 1-heptadecene (3.47 %), octadecyl trifluoroacetate (3.03 %). Additionally, GC-MS detected the presence of compounds in sub-subfraction A2 which were 9,12,15-octadecatrienoic acid, (Z,Z,Z)- (22.21 %), 2-propenoic acid, 3-(2-hydroxyphenyl)-, (E)- (22.06 %), n-hexadecanoic acid (18.89 %), stigmasterol (10.36 %), ?-sitosterol (7.05 %), 1H-inden-1-one, 7-(1,1-dimethylethyl)-2,3- dihydro-3,3-dimethyl (5.15 %), stigmast-7-en-3-ol, (3ß,5a)- (4.48 %), 2H-1-benzopyran, 6,7-dimethoxy-2,2- dimethyl- (3.13 %).
