Numerous microbial populations exist in soil, which contribute to the rhizosphere community's variety through differences in genotype, phenotype and functional traits. These organisms fall under the category of plant growth-promoting rhizobacteria (PGPR), which is a collection of bacteria found in the rhizosphere that supports normal plant growth in the presence of biotic and abiotic stressors by the synthesis of phytohormones, increased enzymatic activity, induction of different PR-proteins, increased nutrients absorption and various other growth promotion processes. In this study, thirty distinct bacteria were isolated from the root and surrounding soil of Solanum lycopersicum L. plants, which were further categorized based on strong fungal antagonistic and distinguished plant growth promotion characteristics. Among these four isolates were selected based on remarkable study outcomes and the 2 best isolates were selected for molecular identification by the Sanger sequencing method. These isolates were identified as Bacillus subtilis and Erwinia tasmaniensis and employed as plant growth-promoting bacteria to support the tomato plant's better growth and high yield.