Effects of allelochemicals from Melaleuca leucadendra (L.) tree leaf leachates on seed germination of Vigna radiata (L.) R. Wilczek
Effects of allelochemicals from Melaleuca leucadendra (L.) tree leaf leachates on seed germination of Vigna radiata (L.) R. Wilczek
- Author(s) J. Hossain, B. Mondal, A. Motaleb1 and P. Maiti
- Year: 2025
- Month: May
- Volume: 65
- Pages: 67-78
We studied the influence of leaf leachates and organic extracts of leaves of exotic tree specie Melaleuca leucadendra (L) on some physiochemical properties of Vigna radiata (L.) Wilczek seeds. The leaf leachate was extracted with organic solvents of decreasing polarity: Methanol > ethyl acetate > chloroform > hexane). Extracts were prepared with each organic solvent at 3-concentrations (5 %, 10 % and 15 %), by adding 5 g, 10 g or 15 g of dried leaf powder to 100 ml of organic solvent. The germination (%), speed of germination and triphenyl tetrazolium chloride stainability were reduced. T50 values (time required for 50 % of seeds to germinate), were increased. These effects were strictly concentration dependent in both leaf leachate and organic extracts. Increased levels of amino acids, soluble carbohydrates and activity of amylase were found. On the other hand, insoluble carbohydrates, proteins and catalase activity were reduced. Methanol extract (15 %) among all organic extracts showed highest inhibitory effects on pre-treated seeds. Through column chromatography the methanol extract was separated into different fractions based on their polarity. Among the isolated fractions Hexane: Ethylacetate (9:1 v/v) elutes showed the maximum inhibition. The High-Resolution Mass Spectroscopic (HRMS) data confirmed the presence of five major compounds viz., neral, 3-methylbutyl heptanoate, nerolidol, geranyl benzoate and phytol in elute
