Maize (Zea mays L.) is a food ingredient in various forms (cornmeal, corn flour, cornstarch, and corn oil. It is also used for processing animal feeds and ethanol). Its seeds were contaminated with aflatoxin B1 by inhibiting seed germination and seedling growth. The inhibitory properties of aflatoxin B1 were determined in the extracts of different bitter plants. A fluctuation in the content of starch, total sugar (TS), reducing sugar (RS) and non-reducing Sugar (NRS) was observed in maize seeds due to treatments of toxin and bitter plants. The maximum starch (62.46), total sugar (0.08) reducing sugar (0.02), and non-reducing sugar (0.028) were found in maize at a 2 ppm concentration of aflatoxin B1. The high levels of starch (14.12), total sugar (1.25), reducing sugar (0.36), and non-reducing sugar (2.46) were recorded in maize seeds due to aflatoxin B1. The a-amylase activity was inhibited by aflatoxin B1 treatment. The maximum O.D. values i.e., 0.68, 0.82, and 0.95 were recorded in maize seeds by treatment of aflatoxin B1 with the extracts of Andrographis paniculata, Azadirachta indica, and Adhatoda vasica.