New centrifuge tube agar profile method to determine the allelopathic effects of plants
New centrifuge tube agar profile method to determine the allelopathic effects of plants
- Author(s) H.N. Yang, C.Y. Zhou, L.M. Wu, Z.R. Li, and L.F. Wang
- Year: 2022
- Month: March
- Volume: 55
- Pages: 151-162
Generally, the laboratory assessment of allelopathy involves the determination of germination rates and measurements of the roots and shoots elongation of receptor plant species exposed to an allelochemical source (e. g. extracts, leachates, root exudates) or a purified allelochemical. However, these assessments are time consuming and laborious. In the present study, a new centrifuge tube agar profile method (CTAP) was introduced to determine the allelopathic effects of aqueous extracts from 30 plant species on lettuce (Lactuca sativa L.). The results demonstrated that the water extract of Melilotus officinalis (L.) Pall. had the strongest inhibitory effects on lettuce. Water extract of M. officinalis (L.) Pall. was analyzed by GC-MS, and the allelopathic effects of coumarin on 5-plant species (Lactuca sativa L., Raphanus sativus L., Raphanus sativus L., Lycopersicon esculentum Mill., Lactuca sativa L., and Brassica campestris var. chinensis) were determined by CTAP. The exposure of coumarin at 100 mg·L-1 for 2 days stimulated the growth of radish (Raphanus sativus L.). This study introduces a new method for the rapid determination of allelopathic potential of plants and allelochemicals.
