Accumulation of allelopathic endogenous hormones and ginsenosides in Panax ginseng in different Models
Accumulation of allelopathic endogenous hormones and ginsenosides in Panax ginseng in different Models
- Author(s) X.R. Meng, Q. Li, E.P. Wang, X. Huang and C.B. Chen
- Year: 2022
- Month: March
- Volume: 55
- Pages: 239-250
In 2-years old ginseng seedlings, growth models of multiple allelopathic pathways were studied in 5-soils in pot culture for 5-months. The endogenous hormones GA, ABA and IAA levels were measured using ELISA assay and 12 ginsenosides were quantified by UPLC-QQQ-MS. We compared the accumulation and correlation between hormone and ginsenosides of PPD (Protopanaxadiol ginsenosides), PPT (Protopanaxatriol ginsenosides) and OLE (Oleanolic acid), types in ginseng under certain allelopathic effects. Compared to the healthy growth model, the leaching, decomposition, exudation and multiple allelopathic pathways combined growth models significantly differed (P < 0.01). Among the leaching, decomposition and exudation, the exudation had the greatest influence on the growth of ginseng. Accumulation of ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2) in ginseng were positively correlated to ABA content and negatively correlated to IAA concentration. The changes in hormone and ginsenosides were highly consistent and showed the correlations between the plant hormones level and ginsenosides accumulation in ginseng. In continuous cropping problem growth model, the combination of multiple allelopathic pathways enhanced the allelopathic effects on the secondary metabolism of ginseng. There were synergistic effects of different allelopathic pathways in continuous cropping problem model to increase the growth inhibition of ginseng.
